In Pictures: Worst Cities for Spring Allergy Sufferers By Angela Haupt March 21, 2012 Comments SHARE 3. Louisville, Ky. Next » 4 of 11 « Back (iStockPhoto) Overall score: 93.31 Louisville lies in the bluegrass region of the state, and grasses are a primary source of pollen. Last year, the city placed 2nd. Next: 4. Jackson, Miss.